Acceleration of incubation processes in DNA bio chips by magnetic particles

Rudolf Heer; Moritz Eggeling; Jörg Schotter; Christa Nöhammer; Rudolf Pichler; Markus Mansfeld; Hubert Brückl

doi:10.1016/j.jmmm.2006.10.1154

Acceleration of incubation processes in DNA bio chips by magnetic particles

Rudolf Heer, Moritz Eggeling, Jörg Schotter, Christa Nöhammer, Rudolf Pichler, Markus Mansfeld, Hubert Brückl

Research output: Contribution to journal › Article › peer-review

Abstract

In classical DNA chip analysis, the target DNA moves by diffusion and Brownian motion only. We introduce a system for enhancing the signals and reducing the hybridization times of bio chips. It allows active agitation within the hybridization buffer by controlled movement of magnetic particles within the analyte solution. First results show that the system easily achieves specific fluorescent signals about four times higher than the ones obtained by a referencing standard procedure within the same hybridization time, while unspecific signals remain unchanged. The device can easily be applied to existing bio chip applications and allows universal operation in the field of molecular diagnostics.

Original language	English
Pages (from-to)	244-248
Journal	Journal of Magnetism and Magnetic Materials
Volume	311
Issue number	1
DOIs	https://doi.org/10.1016/j.jmmm.2006.10.1154
Publication status	Published - 1 Apr 2007
Externally published	Yes

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10.1016/j.jmmm.2006.10.1154

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title = "Acceleration of incubation processes in DNA bio chips by magnetic particles",

abstract = "In classical DNA chip analysis, the target DNA moves by diffusion and Brownian motion only. We introduce a system for enhancing the signals and reducing the hybridization times of bio chips. It allows active agitation within the hybridization buffer by controlled movement of magnetic particles within the analyte solution. First results show that the system easily achieves specific fluorescent signals about four times higher than the ones obtained by a referencing standard procedure within the same hybridization time, while unspecific signals remain unchanged. The device can easily be applied to existing bio chip applications and allows universal operation in the field of molecular diagnostics.",

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AU - Heer, Rudolf

AU - Eggeling, Moritz

AU - Schotter, Jörg

AU - Nöhammer, Christa

AU - Pichler, Rudolf

AU - Mansfeld, Markus

AU - Brückl, Hubert

PY - 2007/4/1

Y1 - 2007/4/1

N2 - In classical DNA chip analysis, the target DNA moves by diffusion and Brownian motion only. We introduce a system for enhancing the signals and reducing the hybridization times of bio chips. It allows active agitation within the hybridization buffer by controlled movement of magnetic particles within the analyte solution. First results show that the system easily achieves specific fluorescent signals about four times higher than the ones obtained by a referencing standard procedure within the same hybridization time, while unspecific signals remain unchanged. The device can easily be applied to existing bio chip applications and allows universal operation in the field of molecular diagnostics.

AB - In classical DNA chip analysis, the target DNA moves by diffusion and Brownian motion only. We introduce a system for enhancing the signals and reducing the hybridization times of bio chips. It allows active agitation within the hybridization buffer by controlled movement of magnetic particles within the analyte solution. First results show that the system easily achieves specific fluorescent signals about four times higher than the ones obtained by a referencing standard procedure within the same hybridization time, while unspecific signals remain unchanged. The device can easily be applied to existing bio chip applications and allows universal operation in the field of molecular diagnostics.

U2 - 10.1016/j.jmmm.2006.10.1154

DO - 10.1016/j.jmmm.2006.10.1154

M3 - Article

SN - 0304-8853

VL - 311

SP - 244

EP - 248

JO - Journal of Magnetism and Magnetic Materials

JF - Journal of Magnetism and Magnetic Materials

IS - 1

ER -

Acceleration of incubation processes in DNA bio chips by magnetic particles

Abstract

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