Abstract
An impedance measurement system with probe signal frequencies up to 50 kHz with AC-probe voltages below 30 mV rms was integrated for wireless and battery-free monitoring of microbiological cell cultures. The here presented modular design and the use of state-of-the-art components greatly eases adoptions to a wide range of biotechnological applications without the need of bulky LCR-meters or potentiostats. The device had a power consumption of less than 2.5 mA at a 3.3 V single power supply and worked trouble-free within the humid environment of a cell culture incubator. Measurements on lumped RC-elements showed an error of less than 1 for absolute values and less than 1 regarding the phase of the complex impedance. The performance of sensor devices with interdigitated electrode structures for the measurement of adherent cell cultures was tested in the presence of phosphate-buffered saline solution in the humid atmosphere of an incubator for biological cell cultures. © 2011 American Institute of Physics.
Original language | English |
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Journal | Review of Scientific Instruments |
Volume | 82 |
Issue number | 11 |
DOIs | |
Publication status | Published - 2011 |
Externally published | Yes |
Keywords
- Absolute values
- Adherent cells
- Biological cells
- Biotechnological applications
- Complex impedance
- Humid atmospheres
- Humid environment
- Impedance measurement
- Impedance measurement techniques
- Inter-digitated electrodes
- Modular designs
- Multi frequency
- Phosphate-buffered saline solutions
- Potentiostats
- Power supply
- Probe signals
- Sensor device
- Cells
- Electric impedance measurement
- Probes
- Sensors
- Voltage regulators
- Cell culture
- article
- culture technique
- impedance
- instrumentation
- microbiological examination
- wireless communication
- Cell Culture Techniques
- Electric Impedance
- Microbiological Techniques
- Wireless Technology