Abstract
Biosensor systems which enable impedance measurements on adherent cell layers under label-free conditions are considered powerful tools for monitoring specific biological characteristics. A radio frequency identification-based sensor platform was adopted to characterize cultivation and differentiation of human bone marrow-derived multipotent stem cells (bmMSC) over periods of up to several days and weeks. Electric cell-substrate impedance sensing was achieved through fabrication of sensitive elements onto glass substrates which comprised two comb-shaped interdigitated gold electrodes covering an area of 1.8 mm × 2. mm. The sensing systems were placed into the wells of a 6-well tissue culture plate, stacked onto a reader unit and could thus be handled and operated under sterile conditions. Continuous measurements were carried out with a sinusoidal voltage of 35. mV at a frequency of 10 kHz. After seeding of human bmMSC, this sensor was able to trace significant impedance changes contingent upon cell spreading and adhesion. The re-usable system was further proven suitable for live examination of cell-substrate attachment or continuous cell monitoring up to several weeks. Induction of either osteogenic or adipogenic differentiation could be validated in bmMSC cultures within a few days, in contrast to state-of-the-art protocols, which require several weeks of cultivation time. In the context of medical cell production in a GMP-compliant process, the here presented interdigitated electric microsensor technology allows the documentation of MSC quality in a fast, efficient and reliable fashion. © 2012 Elsevier B.V.
Original language | English |
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Pages (from-to) | 63-69 |
Number of pages | 7 |
Journal | Biosensors and Bioelectronics |
Volume | 34 |
Issue number | 1 |
DOIs | |
Publication status | Published - 2012 |
Externally published | Yes |
Keywords
- Adherent cells
- Adipogenic
- Bio-sensor systems
- Biological characteristic
- Capacity monitoring
- Cell monitoring
- Cell production
- Cell spreading
- Cell substrates
- Continuous measurements
- Cultivation time
- Glass substrates
- Gold electrodes
- Human bones
- Human mesenchymal stem cells
- Impedance measurement
- Impedance sensing
- Label free
- Osteogenic
- Radio frequencies
- Reader unit
- Sensing systems
- Sensitive elements
- Sensor platform
- Sinusoidal voltage
- Sterile conditions
- Stroma cells
- Tissue culture plates
- Adhesion
- Biosensors
- Cytology
- Differentiation (calculus)
- Electric impedance
- Radio frequency identification (RFID)
- Sensors
- Stem cells
- Tissue culture
- Substrates
- guanosine phosphate
- adipocyte
- article
- biological monitoring
- biosensor
- biotechnology
- bone cell
- cell adhesion
- cell differentiation
- cell spreading
- cellular parameters
- controlled study
- electric impedance sensing
- electric potential
- electrode
- human
- human cell
- measurement
- medical documentation
- mesenchymal stem cell
- multipotent stem cell
- reliability
- tissue culture
- Adipogenesis
- Biosensing Techniques
- Cell Differentiation
- Electric Impedance
- Humans
- Mesenchymal Stem Cells
- Osteogenesis