Acceleration of incubation processes in DNA bio chips by magnetic particles

R. Heer; M. Eggeling; J Schotter; C. Nöhammer; R. Pichler; M. Mansfeld; H. Brückl

doi:10.1016/j.jmmm.2006.10.1154

Acceleration of incubation processes in DNA bio chips by magnetic particles

R. Heer, M. Eggeling, J Schotter, C. Nöhammer, R. Pichler, M. Mansfeld, H. Brückl

Research output: Contribution to journal › Article › peer-review

Abstract

In classical DNA chip analysis, the target DNA moves by diffusion and Brownian motion only. We introduce a system for enhancing the signals and reducing the hybridization times of bio chips. It allows active agitation within the hybridization buffer by controlled movement of magnetic particles within the analyte solution. First results show that the system easily achieves specific fluorescent signals about four times higher than the ones obtained by a referencing standard procedure within the same hybridization time, while unspecific signals remain unchanged. The device can easily be applied to existing bio chip applications and allows universal operation in the field of molecular diagnostics. © 2006 Elsevier B.V. All rights reserved.

Original language	English
Pages (from-to)	244-248
Number of pages	5
Journal	Journal of Magnetism and Magnetic Materials
Volume	311
Issue number	1 SPEC. ISS.
DOIs	https://doi.org/10.1016/j.jmmm.2006.10.1154
Publication status	Published - 2007
Externally published	Yes

Keywords

Brownian movement
DNA
Fluorescence
Liquid films
Magnetic materials
Hybridization buffer
Microparticles
Molecular diagnostics
Biochips

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10.1016/j.jmmm.2006.10.1154

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title = "Acceleration of incubation processes in DNA bio chips by magnetic particles",

abstract = "In classical DNA chip analysis, the target DNA moves by diffusion and Brownian motion only. We introduce a system for enhancing the signals and reducing the hybridization times of bio chips. It allows active agitation within the hybridization buffer by controlled movement of magnetic particles within the analyte solution. First results show that the system easily achieves specific fluorescent signals about four times higher than the ones obtained by a referencing standard procedure within the same hybridization time, while unspecific signals remain unchanged. The device can easily be applied to existing bio chip applications and allows universal operation in the field of molecular diagnostics. {\textcopyright} 2006 Elsevier B.V. All rights reserved.",

keywords = "Brownian movement, DNA, Fluorescence, Liquid films, Magnetic materials, Hybridization buffer, Microparticles, Molecular diagnostics, Biochips",

author = "R. Heer and M. Eggeling and J Schotter and C. N{\"o}hammer and R. Pichler and M. Mansfeld and H. Br{\"u}ckl",

note = "cited By 8",

year = "2007",

doi = "10.1016/j.jmmm.2006.10.1154",

language = "English",

volume = "311",

pages = "244--248",

number = "1 SPEC. ISS.",

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TY - JOUR

T1 - Acceleration of incubation processes in DNA bio chips by magnetic particles

AU - Heer, R.

AU - Eggeling, M.

AU - Schotter, J

AU - Nöhammer, C.

AU - Pichler, R.

AU - Mansfeld, M.

AU - Brückl, H.

N1 - cited By 8

PY - 2007

Y1 - 2007

N2 - In classical DNA chip analysis, the target DNA moves by diffusion and Brownian motion only. We introduce a system for enhancing the signals and reducing the hybridization times of bio chips. It allows active agitation within the hybridization buffer by controlled movement of magnetic particles within the analyte solution. First results show that the system easily achieves specific fluorescent signals about four times higher than the ones obtained by a referencing standard procedure within the same hybridization time, while unspecific signals remain unchanged. The device can easily be applied to existing bio chip applications and allows universal operation in the field of molecular diagnostics. © 2006 Elsevier B.V. All rights reserved.

AB - In classical DNA chip analysis, the target DNA moves by diffusion and Brownian motion only. We introduce a system for enhancing the signals and reducing the hybridization times of bio chips. It allows active agitation within the hybridization buffer by controlled movement of magnetic particles within the analyte solution. First results show that the system easily achieves specific fluorescent signals about four times higher than the ones obtained by a referencing standard procedure within the same hybridization time, while unspecific signals remain unchanged. The device can easily be applied to existing bio chip applications and allows universal operation in the field of molecular diagnostics. © 2006 Elsevier B.V. All rights reserved.

KW - Brownian movement

KW - DNA

KW - Fluorescence

KW - Liquid films

KW - Magnetic materials

KW - Hybridization buffer

KW - Microparticles

KW - Molecular diagnostics

KW - Biochips

U2 - 10.1016/j.jmmm.2006.10.1154

DO - 10.1016/j.jmmm.2006.10.1154

M3 - Article

SN - 0304-8853

VL - 311

SP - 244

EP - 248

JO - Journal of Magnetism and Magnetic Materials

JF - Journal of Magnetism and Magnetic Materials

IS - 1 SPEC. ISS.

ER -

Acceleration of incubation processes in DNA bio chips by magnetic particles

Abstract

Keywords

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