Comparison of a prototype magnetoresistive biosensor to standard fluorescent DNA detection

J Schotter; P.b Kamp; A Becker; A Pühler; G Reiss; H Brückl

doi:10.1016/j.bios.2003.11.007

Comparison of a prototype magnetoresistive biosensor to standard fluorescent DNA detection

J Schotter, P.b Kamp, A Becker, A Pühler, G Reiss, H Brückl

Publikation: Beitrag in Fachzeitschrift › Artikel › Begutachtung

Abstract

We present a comparative analysis of a magnetoresistive biosensor to standard fluorescent DNA detection. The biosensor consists of giant magnetoresistive (GMR) type Cu/Ni80Fe20 multilayers in the second antiferromagnetic coupling maximum. Each of the 206 elements of the magnetoresistive biosensor is patterned into a spiral-shaped line that can cover the area of a typical DNA spot (70 μm diameter). The probe DNA is assembled on top of the sensor elements in different concentrations ranging from 16 pg/μl to 10 ng/μl. Complementary biotin-labeled analyte DNA is hybridized to the probe DNA at a concentration of 10 ng/μl. A number of different commercially available magnetic microspheres are investigated to determine the most appropriate markers. The experimental comparison shows that the relative sensitivity of the magnetoresistive biosensor is superior to the fluorescent detection at low probe DNA concentrations.

Originalsprache	Englisch
Seiten (von - bis)	1149-1156
Fachzeitschrift	Biosensors and Bioelectronics
Jahrgang	19
Ausgabenummer	10
DOIs	https://doi.org/10.1016/j.bios.2003.11.007
Publikationsstatus	Veröffentlicht - 1 Mai 2004
Extern publiziert	Ja

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10.1016/j.bios.2003.11.007

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title = "Comparison of a prototype magnetoresistive biosensor to standard fluorescent DNA detection",

abstract = "We present a comparative analysis of a magnetoresistive biosensor to standard fluorescent DNA detection. The biosensor consists of giant magnetoresistive (GMR) type Cu/Ni80Fe20 multilayers in the second antiferromagnetic coupling maximum. Each of the 206 elements of the magnetoresistive biosensor is patterned into a spiral-shaped line that can cover the area of a typical DNA spot (70 μm diameter). The probe DNA is assembled on top of the sensor elements in different concentrations ranging from 16 pg/μl to 10 ng/μl. Complementary biotin-labeled analyte DNA is hybridized to the probe DNA at a concentration of 10 ng/μl. A number of different commercially available magnetic microspheres are investigated to determine the most appropriate markers. The experimental comparison shows that the relative sensitivity of the magnetoresistive biosensor is superior to the fluorescent detection at low probe DNA concentrations.",

author = "J Schotter and P.b Kamp and A Becker and A P{\"u}hler and G Reiss and H Br{\"u}ckl",

year = "2004",

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AU - Schotter, J

AU - Kamp, P.b

AU - Becker, A

AU - Pühler, A

AU - Reiss, G

AU - Brückl, H

PY - 2004/5/1

Y1 - 2004/5/1

N2 - We present a comparative analysis of a magnetoresistive biosensor to standard fluorescent DNA detection. The biosensor consists of giant magnetoresistive (GMR) type Cu/Ni80Fe20 multilayers in the second antiferromagnetic coupling maximum. Each of the 206 elements of the magnetoresistive biosensor is patterned into a spiral-shaped line that can cover the area of a typical DNA spot (70 μm diameter). The probe DNA is assembled on top of the sensor elements in different concentrations ranging from 16 pg/μl to 10 ng/μl. Complementary biotin-labeled analyte DNA is hybridized to the probe DNA at a concentration of 10 ng/μl. A number of different commercially available magnetic microspheres are investigated to determine the most appropriate markers. The experimental comparison shows that the relative sensitivity of the magnetoresistive biosensor is superior to the fluorescent detection at low probe DNA concentrations.

AB - We present a comparative analysis of a magnetoresistive biosensor to standard fluorescent DNA detection. The biosensor consists of giant magnetoresistive (GMR) type Cu/Ni80Fe20 multilayers in the second antiferromagnetic coupling maximum. Each of the 206 elements of the magnetoresistive biosensor is patterned into a spiral-shaped line that can cover the area of a typical DNA spot (70 μm diameter). The probe DNA is assembled on top of the sensor elements in different concentrations ranging from 16 pg/μl to 10 ng/μl. Complementary biotin-labeled analyte DNA is hybridized to the probe DNA at a concentration of 10 ng/μl. A number of different commercially available magnetic microspheres are investigated to determine the most appropriate markers. The experimental comparison shows that the relative sensitivity of the magnetoresistive biosensor is superior to the fluorescent detection at low probe DNA concentrations.

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DO - 10.1016/j.bios.2003.11.007

M3 - Article

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JO - Biosensors and Bioelectronics

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Comparison of a prototype magnetoresistive biosensor to standard fluorescent DNA detection

Abstract

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