Real time quantitative in-situ DNA hybridization via microcantilever arrays in liquid

Annalisa De Pastina; Giulio Brunetti; Martin Hegner

Real time quantitative in-situ DNA hybridization via microcantilever arrays in liquid

Annalisa De Pastina, Giulio Brunetti, Martin Hegner

Research output: Conference proceeding/Chapter in Book/Report/ › Conference Paper › peer-review

Abstract

This work reports, for the first time, real time quantitative label-free in-situ detection of DNA hybridization via microcantilevers array in liquid. We present a nanomechanical assay based on Silicon microcantilevers array with 18 resonators, enabling sub-nM detection of nucleic acids in liquid environment. The presence of several cantilevers allows differential read out among multiple sensors functionalized with different biomolecules, thus providing internal control towards unspecific binding and environmental variations.

Original language	English
Title of host publication	25th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS2021)
Publication status	Published - Oct 2021
Externally published	Yes

Cite this

@inproceedings{5722905cd06e4ab7a74cc23c11004838,

title = "Real time quantitative in-situ DNA hybridization via microcantilever arrays in liquid",

abstract = "This work reports, for the first time, real time quantitative label-free in-situ detection of DNA hybridization via microcantilevers array in liquid. We present a nanomechanical assay based on Silicon microcantilevers array with 18 resonators, enabling sub-nM detection of nucleic acids in liquid environment. The presence of several cantilevers allows differential read out among multiple sensors functionalized with different biomolecules, thus providing internal control towards unspecific binding and environmental variations.",

author = "{De Pastina}, Annalisa and Giulio Brunetti and Martin Hegner",

year = "2021",

month = oct,

language = "English",

booktitle = "25th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS2021)",

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TY - GEN

T1 - Real time quantitative in-situ DNA hybridization via microcantilever arrays in liquid

AU - De Pastina, Annalisa

AU - Brunetti, Giulio

AU - Hegner, Martin

PY - 2021/10

Y1 - 2021/10

N2 - This work reports, for the first time, real time quantitative label-free in-situ detection of DNA hybridization via microcantilevers array in liquid. We present a nanomechanical assay based on Silicon microcantilevers array with 18 resonators, enabling sub-nM detection of nucleic acids in liquid environment. The presence of several cantilevers allows differential read out among multiple sensors functionalized with different biomolecules, thus providing internal control towards unspecific binding and environmental variations.

AB - This work reports, for the first time, real time quantitative label-free in-situ detection of DNA hybridization via microcantilevers array in liquid. We present a nanomechanical assay based on Silicon microcantilevers array with 18 resonators, enabling sub-nM detection of nucleic acids in liquid environment. The presence of several cantilevers allows differential read out among multiple sensors functionalized with different biomolecules, thus providing internal control towards unspecific binding and environmental variations.

UR - https://microtas2021.org/

M3 - Conference Paper

BT - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS2021)

ER -

Real time quantitative in-situ DNA hybridization via microcantilever arrays in liquid

Abstract

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